Mechanisms of phenazinemediated extracellular electron. Evolution and pathoadaptation of pseudomonas aeruginosa. Pseudomonas aeruginosa burkholderia cepacia which has. Case study of naphalai hospital samut songkhram province by pinit klamklongtun a thesis submitted in partial fulfillment of the requirements for the degree master of science department of environmental science graduate school silpakorn university 2010. The opportunistic pathogen pseudomonas aeruginosa excretes redoxactive small molecules called phenazines. One of the most common pathogens in chronic wounds is pseudomonas aeruginosaa very problematic microbe, due to its ability to formresistant biofilms. Pdf genotypic characterization of clinical isolates of. This is especially true for infections caused by the opportunistic pathogen pseudomonas aeruginosa, with its ability to rapidly develop resistance to multiple classes of antibiotics. Thesis by liang yang infection microbiology group department of systems biology technical university of denmark july 2009 i. It belongs to the newly characterized natural cp inhibitors of the i42 family, designated the icp family. Pseudomonas aeruginosa pathogenicity and antibiotic. The pseudomonas genome database genome annotation and.
Beyond its natural resistance to many drugs, its ability to form biofilm, a complex biological system, renders ineffective the clearance by immune defense systems and antibiotherapy. The work presented in this thesis has found new information regarding the epidemiology of p. The most common resistance mechanisms are downregulation of the porin oprd, and increased activity of multidrug efflux pumps, primarily mexaboprm. Polyvalent inhibitors of pseudomonas aeruginosa adhesion. Pseudomonas aeruginosa iron acquisition in cystic fibrosis. Although the import of resistance mechanisms on mobile genetic elements is always a concern, the most difficult. The antibacterial activities of extracts were evaluated using the disk. Pseudomonas aeruginosa is an opportunistic pathogen that can cause several acute and chronic infections in humans, and it has become an important cause of nosocomial infections and antibiotic resistance. The formation of biofilms by pseudomonas aeruginosa. The large genome, which approaches the complexity and size of lower eukaryotes, and an abundance of regulators, facilitates adaptation to almost any environment.
Thermal deactivation of pseudomonas aeruginosa biofilms. Pseudomonas strains are ubiquitous in soil and water and are able to grow in very simple media at the expense of a great variety of organic compounds. The bacteria produce several virulence factors that allows for establishment in host tissue, where one important substance is the blue pigment pyocyanin. Polyvalent inhibitors of pseudomonas aeruginosa adhesion franz edward boas advised by prof. Pseudomonas aeruginosa an overview sciencedirect topics. Access from the university of nottingham repository. The occurrence of the infection increases with age and can reach 80% in adults behrends et al. Description of bacterial strains used and created in this phd thesis.
Pseudomonas aeruginosa is a gram negative, motile, aerobic rod shaped bacterium which can occur as singles, in pairs and occasionally in short chains. Pdf pseudomonas aeruginosa is an opportunistic human pathogen, responsible for frequently. Penjanaan elektrik yang tinggi ini disumbangkan oleh pyocyanin hasilan. The thesis of this essay is to analyze the mechanisms of action of pseudomonas aeruginosa. Summary treatment of infectious diseases becomes more challenging with each passing year. These drug resistant pathogen has proved challenging for many drug manufacturers because of its mechanisms of action.
Pseudomonas aeruginosa is a common bacterium, gramnegative opportunistic pathogen capable of infecting humans with compromised natural defenses and causing severe pulmonary disease. Evolution of pseudomonas aeruginosa reveals adaptation towards iron acquisition from hemoglobin. Pseudomonas aeruginosa zh1 melalui analisa susunan gen 16s rrna. This thesis provides a description of the population structure of p. Multidrug resistant pseudomonas aeruginosa is one of the major problems facing most countries in fighting pneumonia. The objective of this report is to provide an overview i on p. Pdf pseudomonas aeruginosa pathogenesis and pathogenic.
Pseudomonas aeruginosa is an organism commonly found in the environment, and one of the most common causes of infectious disease in humans. The distribution of pathogenic pseudomonas aeruginosa in sewage showing 14 of 54 pages in this thesis. Pseudomonas aeruginosa inhibitor of cysteine peptidases paicp is a potent protein inhibitor of papainlike cysteine peptidases cps identified in pseudomonas aeruginosa, an opportunistic pathogenic bacteria that can cause severe infections in human. Pseudomonas aeruginosa pseudomonas aeruginosa is a versatile gram negative bacterium that grows in soil, marshes, and coastal marine habitats, as well as on plant and animal tissues. Pseudomonas aeruginosa and nosocomial infections free. Master of science molecular biology, august 2012pages, 22, 1 table. The antibacterial effect of some medicinal plant extracts. Pseudomonas aeruginosa is a gramnegative bacillus found widely in nature, in soil and water.
Comparison of aspartate transcarbamoylase activity between pseudomonas aeruginosa which has one chromosome and burkholderia cepaciawhich has three chromosomes. Ruparell, avika 2012 interplay between quorum sensing. Ferrous iron sensing and responding in pseudomonas aeruginosa. Pseudomonas aeruginosa heinrichheineuniversitat dusseldorf. Ketumpatan kuasa dan ketumpatan arus maksimum yang dijana dalam sfm menggunakan p.
Physiology and mechanisms of pyocyanin reduction in. Thesis prepared for the degree of master of science. Extracellular electron transfer eet, the process whereby cells access electron acceptors or donors that reside many cell lengths away, enables metabolic activity by microorganisms, particularly under oxidantlimited conditions that occur in multicellular bacterial biofilms. Pseudomonas aeruginosa utas eprints university of tasmania. Pseudomonas aeruginosa iron acquisition in cystic fibrosis thesis, doctor of philosophy. Ruparell, avika 2012 interplay between quorum sensing and metabolism in pseudomonas aeruginosa. As a pathogen, it effectively causes disease by acquiring resistance to. Pseudomonas aeruginosa is a ubiquitous extracellular gramnegative bacterium. Multidrug resistant pseudomonas aeruginosa essay examples. Improved confocal imaging and cotreatment with dispersion agents and antibiotics by stacy sommerfeld ross an abstract of a thesis submitted in partial fulfillment of the requirements for the doctor of philosophy degree in pharmacy in the graduate college of the university of iowa may 20. A thesis submitted in partial fulfillment of the requirements for the. Pseudomonas aeruginosa cells were selected for their ability to grow in the presence of 750 ppm alkyldimethylethylbenzyl ammonium chloride qac. Pseudomonas aeruginosa is an opportunistic pathogen that can cause several acute and chronic infections in humans, and it has become an important cause of. Carbapenem imipenem and meropenem resistance in pseudomonas aeruginosa is increasing, with global resistance rates approaching 20%.
Although different mechanisms underpin this process in individual organisms, a potentially widespread strategy involves. Pseudomonas aeruginosa a phenomenon of bacterial resistance tanya strateva 1, daniel yordanov 1. Pdf pathogenic factors of pseudomonas aeruginosa the. Pseudomonas aeruginosa in tasmania open access repository. Thermal deactivation of pseudomonas aeruginosa biofilms by ann marie otoole a thesis submitted in partial fulfillment of the requirements for the master of science degree in chemical and biochemical engineering in the graduate college of the university of iowa may 2015 thesis supervisor. Structure and dynamics of pseudomonas aeruginosa icp.
View pseudomonas aeruginosa research papers on academia. This thesis addresses the possibility that the phenazine pyocyanin acts as an electron acceptor for energy metabolism and exerts beneficial effects on p. A study of clinical strains of pseudomonas aeruginosa and the investigation of antibiotic resistance mechanisms in the multidrug resistant strain pa a thesis submitted to dublin city university in fulfilment of the requirements for the award of the degree of doctor of philosophy by damien ferguson b. Research described in this dissertation focuses on two important aspects of. Research paper pseudomonas aeruginosa is a gramnegative bacteria, particularly known for causing nosocomial infections 1.
Pseudomonas aeruginosa is one of the most common organisms isolated from the respiratory tract of cystic fibrosis patients bendiak and ratjen 2009. Pseudomonas aeruginosa utrecht university repository. The genus pseudomonas is the major one of the family pseudomonadaceae, which comprises a heterogeneous group of rodshaped, gramnegative, polarly flagellated bacteria. A study of clinical strains of pseudomonas aeruginosa and. The extract of medicinal plants were prepared using soxhlet apparatus for alcoholic extract, and water reflux for aqueous extracts. Pseudomonas aeruginosa biofilms thesis presented to the graduate council of texas state universitysan marcos in partial fulfillment of the requirements for the degree master of science by lindsey b. Spatial growth patterns of pseudomonas aeruginosa biofilms by ruifang xu a thesis submitted in partial fulfillment of the requirements for the degree of master of science in chemical engineering montana state university bozeman, montana july 2004. Draft genome sequences of pseudomonas aeruginosa b3. Pseudomonas aeruginosa bacterial biofilms by charlotte pye a thesis presented to the university of guelph in partial fulfilment of requirements for the degree of doctor of veterinary science in clinical studies guelph, ontario, canada charlotte pye, august 20. Pseudomonas aeruginosa charles river research animal. Pseudomonas aeruginosa colonization in patients with cystic fibrosis. This thesis explores a strategy which aims to counteract p. People with cystic fibrosis, burn victims, individuals with cancer, and persons infected with hiv are particularly at risk of disease resulting from pseudomonas.